DNA Cloning Service

Binaries

p7oAct-LH B489

Ligation of A373p6ido-Act* into B474p7oM-LH*

12345678910pVS1ACTSm/SpColE1barTE9barintLBRBB489p7oAct-LH10,187 bp
Promoter 1
Act
Gene 1
bar
Features
monocot
Size
10,187 bp, circular
Record date
22-JAN-2016

Files

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Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
LB T-DNA border misc_feature 12424 +
ACT Promoter misc_feature 3131,7101,398 +
bar Selection marker CDS 1,7381,971234 +
int Site misc_feature 1,9722,160189 +
bar Selection marker CDS 2,1612,478318 +
TE9 Terminator misc_feature 2,4982,788291 +
RB T-DNA border misc_feature 2,9642,98724 +
ColE1 Origin / backbone misc_feature 3,5224,219698 +
pVS1 Origin / backbone misc_feature 4,7688,4733,706 +
Sm/Sp Selection marker CDS 8,6579,6671,011 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.