p7oUZm-ActGFP B599
Ligation of B596pActOs-mGFPM-ocs* into B479p7oUZm-LH*
- Promoter 1
- UbiZm
- Gene 1
- bar
- Promoter 2
- Act
- Gene 2
- EGFP
- Features
- monocot
- Size
- 12,794 bp, circular
- Record date
- 26-JAN-2016
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| UbiZm | Coding sequence CDS | 62 | 1,569 | 1,508 | + |
| int | Site misc_feature | 1,049 | 1,568 | 520 | + |
| bar | Selection marker CDS | 1,590 | 1,823 | 234 | + |
| int | Site misc_feature | 1,824 | 2,012 | 189 | + |
| bar | Selection marker CDS | 2,013 | 2,330 | 318 | + |
| TE9 | Terminator misc_feature | 2,350 | 2,640 | 291 | + |
| ACT | Promoter misc_feature | 2,707 | 4,103 | 1,397 | + |
| gfp | Coding sequence CDS | 4,129 | 4,507 | 379 | + |
| int | Site CDS | 4,508 | 4,696 | 189 | + |
| gfp | Coding sequence CDS | 4,697 | 5,034 | 338 | + |
| Tocs | Terminator misc_feature | 5,043 | 5,238 | 196 | + |
| RB | T-DNA border misc_feature | 5,323 | 5,346 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 5,881 | 6,578 | 698 | + |
| pVS1 | Origin / backbone misc_feature | 7,127 | 10,832 | 3,706 | + |
| Sm/Sp | Selection marker CDS | 11,016 | 12,026 | 1,011 | + |
| LB | T-DNA border misc_feature | 12,547 | 12,570 | 24 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.