DNA Cloning Service

Binaries

p9o-LH-U10-ocs C599

Ligation of 204pUbiAtmu-ocs* into B415p9o-LH*

1234567891011pVS1Pro-Ubq10AtSm/SpColE1NptIIo35SIntNptIIoE9bomOCSIntRBLBC599p9o-LH-U10-o11,113 bp
Promoter 1
35s
Gene 1
nptII
Promoter 2
Ubq10
Gene 2
MCS
Features
dicots
Size
11,113 bp, circular
Record date
29-SEP-2017

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
LB T-DNA border misc_feature 12424 +
35S Promoter misc_feature 309724416 +
NptIIo Selection marker CDS 7451,043299 +
splice Site misc_feature 1,0401,0434 +
Int Site CDS 1,0441,230187 +
splice Site misc_feature 1,2291,2324 +
NptIIo Selection marker CDS 1,2311,734504 +
E9 Terminator misc_feature 1,7482,038291 +
Pro-Ubq10At Promoter misc_feature 2,0873,5621,476 +
Int Site misc_feature 3,2573,560304 +
OCS Terminator misc_feature 3,6103,805196 +
RB T-DNA border misc_feature 3,8903,91324 +
ColE1 Origin / backbone misc_feature 4,4485,145698 +
bom Origin / backbone misc_feature 5,1825,441260 +
pVS1 Origin / backbone misc_feature 5,6949,3993,706 +
Sm/Sp Selection marker CDS 9,58310,5931,011 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.