p9o-UbiAt-DoGus C204
Ligation of B494pUbiAt-DoGusi-ocs* into B415p9o-LH*
- Promoter 1
- 35s
- Gene 1
- nptII
- Promoter 2
- Ubq10
- Gene 2
- oGus
- Features
- dicots
- Size
- 13,115 bp, circular
- Record date
- 29-SEP-2017
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| LB | T-DNA border misc_feature | 1 | 24 | 24 | + |
| 35S | Promoter misc_feature | 309 | 724 | 416 | + |
| NptIIo | Selection marker CDS | 745 | 1,043 | 299 | + |
| splice | Site misc_feature | 1,040 | 1,043 | 4 | + |
| Int | Site CDS | 1,044 | 1,230 | 187 | + |
| splice | Site misc_feature | 1,229 | 1,232 | 4 | + |
| NptIIo | Selection marker CDS | 1,231 | 1,734 | 504 | + |
| E9 | Terminator misc_feature | 1,748 | 2,038 | 291 | + |
| Pro-Ubq10At | Promoter misc_feature | 2,105 | 3,580 | 1,476 | + |
| Int | Site misc_feature | 3,275 | 3,578 | 304 | + |
| ogus | Coding sequence CDS | 3,606 | 4,220 | 615 | + |
| 'Intron | Site misc_feature | 4,221 | 4,409 | 189 | + |
| ogus | Coding sequence CDS | 4,410 | 5,606 | 1,197 | + |
| OCS | Terminator misc_feature | 5,612 | 5,807 | 196 | + |
| RB | T-DNA border misc_feature | 5,892 | 5,915 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 6,450 | 7,147 | 698 | + |
| bom | Origin / backbone misc_feature | 7,184 | 7,443 | 260 | + |
| pVS1 | Origin / backbone misc_feature | 7,696 | 11,401 | 3,706 | + |
| Sm/Sp | Selection marker CDS | 11,585 | 12,595 | 1,011 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.