p9o-UbiAtGFP B891
Ligation of B592pUbiAt-mGFPD-ocs* into B415p9o-LH*
- Promoter 1
- 35s
- Gene 1
- nptII
- Promoter 2
- Ubq10
- Gene 2
- EGFP
- Features
- dicots
- Size
- 12,023 bp, circular
- Record date
- 01-FEB-2021
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| LB | T-DNA border misc_feature | 1 | 24 | 24 | + |
| P35S | Promoter misc_feature | 309 | 724 | 416 | + |
| nptIIo | Selection marker CDS | 745 | 1,043 | 299 | + |
| int | Site CDS | 1,044 | 1,230 | 187 | + |
| nptIIo | Selection marker CDS | 1,231 | 1,734 | 504 | + |
| TE9 | Terminator misc_feature | 1,748 | 2,038 | 291 | + |
| Pro-Ubq10At | Promoter misc_feature | 2,105 | 3,580 | 1,476 | + |
| TATA | Site misc_feature | 3,176 | 3,179 | 4 | + |
| Ubi cds 5` | Coding sequence CDS | 3,209 | 3,274 | 66 | + |
| int | Site misc_feature | 3,275 | 3,578 | 304 | + |
| EGFP | Coding sequence CDS | 3,606 | 3,984 | 379 | + |
| int | Site CDS | 3,985 | 4,173 | 189 | + |
| EGFP | Coding sequence CDS | 4,174 | 4,511 | 338 | + |
| OCS | Terminator misc_feature | 4,520 | 4,715 | 196 | + |
| RB | T-DNA border misc_feature | 4,800 | 4,823 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 5,358 | 6,055 | 698 | + |
| bom | Origin / backbone misc_feature | 6,092 | 6,351 | 260 | + |
| pVS1 | Origin / backbone misc_feature | 6,604 | 10,309 | 3,706 | + |
| Sm/Sp | Selection marker misc_feature | 10,493 | 11,503 | 1,011 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.