DNA Cloning Service

Binaries

p9o2x35s-LH B485

Ligation of 35s-Xba-xho* into B475p9oM-LH*

123456789pVS1Sm/SpP2x35sColE1nptIIonptIIoTE9intLBRBB485p9o2x35s-LH9,815 bp
Promoter 1
2x35s
Gene 1
nptII
Features
monocot
Size
9,815 bp, circular
Record date
22-JAN-2016

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
LB T-DNA border misc_feature 12424 +
P2x35s Promoter misc_feature 3141,098785 +
nptIIo Selection marker CDS 1,1161,414299 +
int Site CDS 1,4151,601187 +
nptIIo Selection marker CDS 1,6022,108507 +
TE9 Terminator misc_feature 2,1262,416291 +
RB T-DNA border misc_feature 2,5922,61524 +
ColE1 Origin / backbone misc_feature 3,1503,847698 +
pVS1 Origin / backbone misc_feature 4,3968,1013,706 +
Sm/Sp Selection marker CDS 8,2859,2951,011 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.