DNA Cloning Service

Binaries

p9oN-35s-ocs-LH B927

Ligation of p35-OCS-BM* into B401p9oN-LH*

123456789pVS1 ORISm/SpColE1''NptIIo'35S-ProNptIIo'P-NOSE9OCS'intronB927p9oN-35s-ocs9,945 bp
Promoter 1
Nos
Gene 1
nptII
Promoter 2
35s
Gene 2
MCS
Features
dicots
Size
9,945 bp, circular
Record date
29-SEP-2017

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
BL Site misc_feature 12424 +
P-NOS Other misc_feature 309606298 +
NptIIo' Selection marker CDS 625923299 +
splice Site misc_feature 9209234 +
'intron Coding sequence CDS 9241,110187 +
splice Site misc_feature 1,1091,1124 +
''NptIIo' Selection marker CDS 1,1111,614504 +
E9 Terminator misc_feature 1,6281,918291 +
35S-Pro Promoter misc_feature 1,9732,395423 +
OCS Terminator misc_feature 2,4422,637196 +
BR Site misc_feature 2,7222,74524 +
ColE1 Origin / backbone misc_feature 3,2803,977698 +
pVS1 ORI Origin / backbone misc_feature 4,5268,2313,706 +
Sm/Sp Selection marker CDS 8,4159,4251,011 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.