p9oU10-oGUS-int B441

Ligation of B458pDoGusi-ocs* into B435p9oU10-LH*

Files

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Feature	Type	Start	End	Length	Strand
LB	T-DNA border misc_feature	1	24	24	+
UBQ10	Other misc_feature	309	1,784	1,476	+
Ubi cds 5	Promoter misc_feature	1,413	1,476	64	+
int	Site misc_feature	1,479	1,782	304	+
nptIIo	Selection marker CDS	1,804	2,102	299	+
splice	Site misc_signal	2,099	2,102	4	+
int	Site CDS	2,103	2,289	187	+
splice	Site misc_signal	2,288	2,291	4	+
nptIIo	Selection marker CDS	2,290	2,793	504	+
TE9	Terminator misc_feature	2,807	3,097	291	+
ogus	Coding sequence CDS	3,199	3,813	615	+
int	Site misc_feature	3,814	4,002	189	+
ogus	Coding sequence CDS	4,003	5,199	1,197	+
Tocs	Terminator misc_feature	5,205	5,400	196	+
RB	T-DNA border misc_feature	5,485	5,508	24	+
ColE1	Origin / backbone misc_feature	6,043	6,740	698	+
bom	Origin / backbone misc_feature	6,777	7,036	260	+
pVS1	Origin / backbone misc_feature	7,289	10,994	3,706	+
Sm/Sp	Selection marker CDS	11,178	12,188	1,011	+

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.