DNA Cloning Service

Binaries

p9oU10-ocs-LH B448

Ligation of pOCS-BM* into B435p9oU10-LH*

12345678910pVS1UBQ10Sm/SpColE1nptIIointnptIIoTE9bomTocsintUbi cds 5LBRBB448p9oU10-ocs-L10,696 bp
Promoter 1
Ubq10
Gene 1
nptII
Gene 2
MCS
Features
dicots
Size
10,696 bp, circular
Record date
22-JAN-2016

Files

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Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
LB T-DNA border misc_feature 12424 +
UBQ10 Other misc_feature 3091,7841,476 +
Ubi cds 5 Promoter misc_feature 1,4131,47664 +
int Site misc_feature 1,4791,782304 +
nptIIo Selection marker CDS 1,8042,102299 +
splice Site misc_signal 2,0992,1024 +
int Site CDS 2,1032,289187 +
splice Site misc_signal 2,2882,2914 +
nptIIo Selection marker CDS 2,2902,793504 +
TE9 Terminator misc_feature 2,8073,097291 +
Tocs Terminator misc_feature 3,1933,388196 +
RB T-DNA border misc_feature 3,4733,49624 +
ColE1 Origin / backbone misc_feature 4,0314,728698 +
bom Origin / backbone misc_feature 4,7655,024260 +
pVS1 Origin / backbone misc_feature 5,2778,9823,706 +
Sm/Sp Selection marker CDS 9,16610,1761,011 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.