p9oUH-LH-ocs B421

Ligation of pOCS-BM* into B418p9oUH-LH*

Files

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Feature	Type	Start	End	Length	Strand
LB	T-DNA border misc_feature	1	24	24	+
UbiZm	Promoter misc_feature	316	651	336	+
cat	Site misc_signal	433	435	3	+
cat	Site misc_signal	436	438	3	+
cat	Site misc_signal	469	471	3	+
cat	Site misc_signal	498	500	3	+
cat	Site misc_signal	509	511	3	+
cat	Site misc_signal	530	532	3	+
cat	Site misc_signal	533	535	3	+
tata	Site misc_signal	538	541	4	+
mRNA	Coding sequence CDS	569	651	83	+
nptIIo	Selection marker CDS	671	969	299	+
splice	Site misc_signal	966	969	4	+
int	Site CDS	970	1,156	187	+
splice	Site misc_signal	1,155	1,158	4	+
nptIIo	Selection marker CDS	1,157	1,660	504	+
TE9	Terminator misc_feature	1,674	1,964	291	+
Tocs	Terminator misc_feature	2,060	2,255	196	+
RB	T-DNA border misc_feature	2,340	2,363	24	+
ColE1	Origin / backbone misc_feature	2,898	3,595	698	+
bom	Origin / backbone misc_feature	3,632	3,891	260	+
pVS1	Origin / backbone misc_feature	4,144	7,849	3,706	+
Sm/Sp	Selection marker CDS	8,033	9,043	1,011	+

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.