p6i2xoR-Ucas-U6Os2 B524

Ligation of Ubi-Cas-Sbf-Nru* into B328p6i2xoR-Ucas-U6Os*

Files

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Feature	Type	Start	End	Length	Strand
P2x35s	Promoter misc_feature	67	814	748	+
tata	Site misc_feature	781	784	4	+
5´UTR	Site misc_feature	815	850	36	+
Kozak	Site misc_feature	867	871	5	+
hptII	Selection marker CDS	872	1,441	570	+
int	Site misc_feature	1,442	1,630	189	+
'hptII	Selection marker CDS	1,631	2,086	456	+
E9	Terminator misc_feature	2,097	2,387	291	+
transcription stop signal	Site misc_feature	2,191	2,196	6	+
transcription stop signal	Site misc_feature	2,197	2,202	6	+
transcription stop signal	Site misc_feature	2,215	2,220	6	+
Ubi-int	Promoter misc_feature	2,442	3,950	1,509	+
Intron	Site misc_feature	3,429	3,948	520	+
'Cas9	Coding sequence CDS	3,969	5,419	1,451	+
D10A	Site misc_feature	4,045	4,045	1	+
int	Site misc_feature	4,392	4,484	93	+
ORF-1'	Coding sequence CDS	4,590	5,419	830	+
'Cas9	Coding sequence CDS	5,420	8,264	2,845	+
NLS	Site misc_feature	8,214	8,261	48	+
NOS-T	Terminator misc_feature	8,270	8,523	254	+
U6Os	Promoter misc_feature	8,533	8,985	453	+
MCS	Site CDS	8,987	9,012	26	+
hairpin	Site CDS	9,013	9,042	30	+
gRNA	Site misc_feature	9,013	9,095	83	+
RB	T-DNA border misc_feature	9,181	9,204	24	+
ColE1	Origin / backbone misc_feature	9,739	10,436	698	+
'pVS1'	Origin / backbone misc_feature	10,724	13,511	2,788	+
Sm/Sp	Selection marker CDS	13,695	14,705	1,011	+
LB	T-DNA border misc_feature	15,226	15,249	24	+

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.