p6ioR-35sCasWToi B792
Ligation of B413p6o-LH* into B764p9ioR-35sCasWToi*
- Promoter 1
- 35s
- Gene 1
- hptII
- Promoter 2
- 35s
- Gene 2
- Cas9
- Features
- U6At
- Size
- 13,907 bp, circular
- Record date
- 31-MAR-2016
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| LB | T-DNA border misc_feature | 1 | 24 | 24 | + |
| 35S | Promoter misc_feature | 309 | 724 | 416 | + |
| Kozak | Site misc_feature | 740 | 744 | 5 | + |
| hptII | Selection marker CDS | 745 | 1,314 | 570 | + |
| int | Site misc_feature | 1,315 | 1,503 | 189 | + |
| 'hptII | Selection marker CDS | 1,504 | 1,959 | 456 | + |
| 'E9 | Terminator misc_feature | 1,970 | 2,260 | 291 | + |
| 35S-Pro | Promoter misc_feature | 2,315 | 2,732 | 418 | + |
| TATA-box | Site CDS | 2,703 | 2,709 | 7 | + |
| 'Cas9WT | Coding sequence CDS | 2,758 | 6,954 | 4,197 | + |
| NLS | Site misc_feature | 2,764 | 2,784 | 21 | + |
| NLS | Site misc_feature | 6,904 | 6,951 | 48 | + |
| NOS-T | Terminator misc_feature | 6,960 | 7,213 | 254 | + |
| 'RNA' | Other misc_feature | 7,218 | 7,778 | 561 | + |
| U6At | Coding sequence CDS | 7,223 | 7,667 | 445 | + |
| targetRNA | Site CDS | 7,668 | 7,694 | 27 | + |
| hairpin | Site CDS | 7,695 | 7,724 | 30 | + |
| gRNA | Site misc_feature | 7,695 | 7,777 | 83 | + |
| RB | T-DNA border misc_feature | 7,863 | 7,886 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 8,421 | 9,118 | 698 | + |
| 'pVS1 | Origin / backbone misc_feature | 9,406 | 12,193 | 2,788 | + |
| Sm/Sp | Selection marker CDS | 12,377 | 13,387 | 1,011 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.