p6ioRAct-UZmCwtoi C143

Ligation of C164p7ioR-UZmCwtoi-Bbs* into C141p6ioRAct-LH*

Files

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Feature	Type	Start	End	Length	Strand
LB	T-DNA border misc_feature	1	24	24	+
pAct	Promoter misc_feature	313	1,710	1,398	+
del	Coding sequence CDS	1,140	1,268	129	+
Kozak	Site misc_feature	1,733	1,737	5	+
hptII	Selection marker CDS	1,738	2,307	570	+
int	Site misc_feature	2,308	2,496	189	+
'hptII	Selection marker CDS	2,497	2,952	456	+
'E9	Terminator misc_feature	2,970	3,260	291	+
Ubi-int	Promoter misc_feature	3,315	4,823	1,509	+
Intron	Site misc_feature	4,302	4,821	520	+
NLS	Site misc_feature	4,854	4,874	21	+
'Cas9WT	Coding sequence CDS	4,890	9,044	4,155	+
NLS	Site misc_feature	8,994	9,041	48	+
NOS-T	Terminator misc_feature	9,050	9,303	254	+
U6Os'	Promoter misc_feature	9,313	9,765	453	+
insert target RNA	Site CDS	9,766	9,794	29	+
hairpin	Site CDS	9,795	9,824	30	+
gRNA scaffold	Site misc_feature	9,795	9,877	83	+
RB	T-DNA border misc_feature	9,963	9,986	24	+
ColE1	Origin / backbone misc_feature	10,521	11,218	698	+
'pVS1	Origin / backbone misc_feature	11,506	14,293	2,788	+
Sm/Sp	Selection marker CDS	14,477	15,487	1,011	+

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.