p7D35s-C9wt-U6Gm D024

Ligation of D026pHC-U6Gm* into D023p7D35s-C9wt-U6At*

Files

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Feature	Type	Start	End	Length	Strand
LB'	T-DNA border misc_feature	1	24	24	+
P35S	Promoter misc_feature	169	584	416	+
Bar	Selection marker CDS	605	838	234	+
STLS1	Other misc_feature	839	1,027	189	+
Bar	Selection marker CDS	1,028	1,343	316	+
TE9	Terminator misc_feature	1,356	1,646	291	+
Pro-Ubq10At	Promoter misc_feature	1,672	3,147	1,476	+
Ubi cds 5	Promoter misc_feature	2,776	2,839	64	+
íntron	Other misc_feature	2,842	3,145	304	+
'Cas9WT	Coding sequence CDS	3,169	7,365	4,197	+
NLS	Site misc_feature	3,175	3,195	21	+
NLS	Site misc_feature	7,315	7,362	48	+
NOS-T	Terminator misc_feature	7,371	7,624	254	+
U6Gm	Promoter misc_feature	7,634	7,938	305	+
targetRNA	Site CDS	7,939	7,965	27	+
hairpin	Site CDS	7,966	7,995	30	+
gRNA	Site misc_feature	7,966	8,048	83	+
Right Border Region	Other misc_feature	8,089	8,691	603	+
RB	T-DNA border misc_feature	8,134	8,157	24	+
ColE1	Origin / backbone misc_feature	8,705	9,363	659	+
''pVS1 ORI'''	Origin / backbone misc_feature	9,392	11,355	1,964	+
ParB	Coding sequence CDS	9,718	10,893	1,176	+
strep/spec	Selection marker CDS	11,544	12,554	1,011	+
Left Border Region	T-DNA border misc_feature	12,622	163	0	+

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.