p7i-Ucas-U6Os B349
Ligation of Frag-BstE-Aar* into B329p7i2xoR-Ucas-U6Os*corrected
- Promoter 1
- 2x35s
- Gene 1
- bar
- Promoter 2
- UbiZm
- Gene 2
- Cas9 nick
- Features
- monocots
- Size
- 14,992 bp, circular
- Record date
- 12-JUL-2018
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| LB | T-DNA border misc_feature | 1 | 24 | 24 | + |
| P2x35s' | Promoter misc_feature | 315 | 1,099 | 785 | + |
| bar | Selection marker CDS | 1,113 | 1,346 | 234 | + |
| STLS1 | Other misc_feature | 1,347 | 1,535 | 189 | + |
| bar' | Selection marker CDS | 1,536 | 1,853 | 318 | + |
| E9 | Terminator misc_feature | 1,864 | 2,154 | 291 | + |
| Ubi-int | Promoter misc_feature | 2,209 | 3,717 | 1,509 | + |
| Intron | Site misc_feature | 3,196 | 3,715 | 520 | + |
| 'Cas9 | Coding sequence CDS | 3,743 | 8,031 | 4,289 | + |
| NLS | Site misc_feature | 3,748 | 3,768 | 21 | + |
| D10A | Site misc_feature | 3,812 | 3,812 | 1 | + |
| int | Site misc_feature | 4,159 | 4,251 | 93 | + |
| NLS | Site misc_feature | 7,981 | 8,028 | 48 | + |
| NOS-T | Terminator misc_feature | 8,037 | 8,290 | 254 | + |
| PU6C4 | Promoter misc_feature | 8,300 | 8,752 | 453 | + |
| MCS | Site CDS | 8,754 | 8,779 | 26 | + |
| hairpin | Site CDS | 8,780 | 8,809 | 30 | + |
| gRNA | Site misc_feature | 8,780 | 8,862 | 83 | + |
| RB | T-DNA border misc_feature | 8,948 | 8,971 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 9,506 | 10,203 | 698 | + |
| 'pVS1' | Origin / backbone misc_feature | 10,491 | 13,278 | 2,788 | + |
| Sm/Sp' | Selection marker CDS | 13,462 | 14,472 | 1,011 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.