p7i2x-Ucas-U6Os2 B525
Ligation of B524p6i2xoR-Ucas-U6Os2* into B349p7i-Ucas-U6Os*
- Promoter 1
- 2x35s
- Gene 1
- bar
- Promoter 2
- UbiZm
- Gene 2
- Cas9 nick
- Features
- monocots
- Size
- 14,998 bp, circular
- Record date
- 29-AUG-2016
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| LB | T-DNA border misc_feature | 1 | 24 | 24 | + |
| P2x35s' | Promoter misc_feature | 321 | 1,105 | 785 | + |
| bar' | Selection marker misc_feature | 1,119 | 1,352 | 234 | + |
| STLS1 | Other misc_feature | 1,353 | 1,541 | 189 | + |
| ''bar | Selection marker misc_feature | 1,542 | 1,859 | 318 | + |
| 'E9 | Terminator misc_feature | 1,870 | 2,160 | 291 | + |
| Ubi-int | Promoter misc_feature | 2,215 | 3,723 | 1,509 | + |
| Intron | Site misc_feature | 3,202 | 3,721 | 520 | + |
| 'Cas9 | Coding sequence CDS | 3,742 | 8,037 | 4,296 | + |
| NLS | Site misc_feature | 3,748 | 3,768 | 21 | + |
| link | Site misc_feature | 3,769 | 3,792 | 24 | + |
| D10A | Site misc_feature | 3,818 | 3,818 | 1 | + |
| int | Site misc_feature | 4,165 | 4,257 | 93 | + |
| NLS | Site misc_feature | 7,987 | 8,034 | 48 | + |
| NOS-T | Terminator misc_feature | 8,043 | 8,296 | 254 | + |
| PU6C4 | Promoter misc_feature | 8,306 | 8,758 | 453 | + |
| 'targetRNA | Site CDS | 8,760 | 8,785 | 26 | + |
| hairpin | Site CDS | 8,786 | 8,815 | 30 | + |
| gRNA | Site misc_feature | 8,786 | 8,868 | 83 | + |
| RB | T-DNA border misc_feature | 8,954 | 8,977 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 9,512 | 10,209 | 698 | + |
| 'pVS1 | Origin / backbone misc_feature | 10,497 | 13,284 | 2,788 | + |
| Sm/Sp | Selection marker CDS | 13,468 | 14,478 | 1,011 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.