p7ioR-35sCasD10A B396
Ligation of B353p7im2* into B358p9ioR-35sCasD10A*
- Promoter 1
- 35s
- Gene 1
- bar
- Promoter 2
- 35s
- Gene 2
- Cas9 nick
- Features
- U6At
- Size
- 13,528 bp, circular
- Record date
- 13-MAY-2015
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| LB | T-DNA border misc_feature | 1 | 24 | 24 | + |
| 35S | Promoter misc_feature | 309 | 724 | 416 | + |
| bar' | Selection marker CDS | 741 | 974 | 234 | + |
| STLS1 | Coding sequence CDS | 975 | 1,163 | 189 | + |
| ''bar | Selection marker CDS | 1,164 | 1,481 | 318 | + |
| 'E9 | Terminator misc_feature | 1,492 | 1,782 | 291 | + |
| 35S-Pro | Promoter misc_feature | 1,837 | 2,254 | 418 | + |
| TATA-box | Site CDS | 2,225 | 2,231 | 7 | + |
| 'Cas9 | Coding sequence CDS | 2,280 | 6,575 | 4,296 | + |
| NLS | Site misc_feature | 2,292 | 2,312 | 21 | + |
| D10A | Site misc_feature | 2,356 | 2,356 | 1 | + |
| int | Site misc_feature | 2,703 | 2,795 | 93 | + |
| NLS | Site misc_feature | 6,525 | 6,572 | 48 | + |
| NOS-T | Terminator misc_feature | 6,581 | 6,834 | 254 | + |
| U6At | Coding sequence CDS | 6,844 | 7,288 | 445 | + |
| targetRNA | Site CDS | 7,289 | 7,315 | 27 | + |
| hairpin | Site CDS | 7,316 | 7,345 | 30 | + |
| gRNA | Site misc_feature | 7,316 | 7,398 | 83 | + |
| RB | T-DNA border misc_feature | 7,484 | 7,507 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 8,042 | 8,739 | 698 | + |
| 'pVS1 | Origin / backbone misc_feature | 9,027 | 11,814 | 2,788 | + |
| Sm/Sp | Selection marker CDS | 11,998 | 13,008 | 1,011 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.