p7ioR-35sCasWToi C034
Ligation of B414p7o-LH* into B792p6ioR-35sCasWToi*
- Promoter 1
- 35s
- Gene 1
- bar
- Promoter 2
- 35s
- Gene 2
- Cas9
- Features
- U6At
- Size
- 13,433 bp, circular
- Record date
- 14-JUL-2016
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| LB | T-DNA border misc_feature | 1 | 24 | 24 | + |
| 35S | Promoter misc_feature | 309 | 724 | 416 | + |
| Bar' | Selection marker CDS | 745 | 978 | 234 | + |
| STLS1 | Other misc_feature | 979 | 1,167 | 189 | + |
| ''Bar' | Selection marker CDS | 1,168 | 1,483 | 316 | + |
| 'E9 | Terminator misc_feature | 1,496 | 1,786 | 291 | + |
| 35S-Pro | Promoter misc_feature | 1,841 | 2,258 | 418 | + |
| TATA-box | Site CDS | 2,229 | 2,235 | 7 | + |
| 'Cas9WT | Coding sequence CDS | 2,284 | 6,480 | 4,197 | + |
| NLS | Site misc_feature | 2,290 | 2,310 | 21 | + |
| NLS | Site misc_feature | 6,430 | 6,477 | 48 | + |
| NOS-T | Terminator misc_feature | 6,486 | 6,739 | 254 | + |
| 'RNA' | Other misc_feature | 6,744 | 7,304 | 561 | + |
| U6At | Coding sequence CDS | 6,749 | 7,193 | 445 | + |
| targetRNA | Site CDS | 7,194 | 7,220 | 27 | + |
| hairpin | Site CDS | 7,221 | 7,250 | 30 | + |
| gRNA | Site misc_feature | 7,221 | 7,303 | 83 | + |
| RB | T-DNA border misc_feature | 7,389 | 7,412 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 7,947 | 8,644 | 698 | + |
| 'pVS1 | Origin / backbone misc_feature | 8,932 | 11,719 | 2,788 | + |
| Sm/Sp | Selection marker CDS | 11,903 | 12,913 | 1,011 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.