p7ioR-UZmCwtoi B819
Ligation of B792p6ioR-35sCasWToi* into B350p7i-UcasW-U6Os*
- Promoter 1
- 2x35s
- Gene 1
- bar
- Promoter 2
- UbiZm
- Gene 2
- Cas9
- Features
- monocots
- Size
- 14,903 bp, circular
- Record date
- 15-JUN-2016
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| LB | T-DNA border misc_feature | 1 | 24 | 24 | + |
| P2x35s' | Promoter misc_feature | 315 | 1,099 | 785 | + |
| bar | Selection marker CDS | 1,113 | 1,346 | 234 | + |
| STLS1 | Other misc_feature | 1,347 | 1,535 | 189 | + |
| bar' | Selection marker CDS | 1,536 | 1,853 | 318 | + |
| E9 | Terminator misc_feature | 1,868 | 2,158 | 291 | + |
| Ubi-int | Promoter misc_feature | 2,213 | 3,721 | 1,509 | + |
| Intron | Site misc_feature | 3,200 | 3,719 | 520 | + |
| NLS | Site misc_feature | 3,752 | 3,772 | 21 | + |
| 'Cas9WT | Coding sequence CDS | 3,788 | 7,942 | 4,155 | + |
| NLS | Site misc_feature | 7,892 | 7,939 | 48 | + |
| NOS-T | Terminator misc_feature | 7,948 | 8,201 | 254 | + |
| U6Os | Promoter misc_feature | 8,211 | 8,663 | 453 | + |
| targetRNA | Site CDS | 8,675 | 8,690 | 16 | + |
| hairpin | Site CDS | 8,691 | 8,720 | 30 | + |
| gRNA scaffold | Site misc_feature | 8,691 | 8,773 | 83 | + |
| RB | T-DNA border misc_feature | 8,859 | 8,882 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 9,417 | 10,114 | 698 | + |
| 'pVS1' | Origin / backbone misc_feature | 10,402 | 13,189 | 2,788 | + |
| Sm/Sp' | Selection marker CDS | 13,373 | 14,383 | 1,011 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.