p7ioR-Uq10CasWToi C035
Ligation of A390p9i-UbiAtm-ogusi* into C034p7ioR-35sCasWToi*
- Promoter 1
- 35s
- Gene 1
- bar
- Promoter 2
- UbiAt
- Gene 2
- Cas9
- Features
- U6At
- Size
- 14,481 bp, circular
- Record date
- 21-FEB-2017
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| LB | T-DNA border misc_feature | 1 | 24 | 24 | + |
| 35S | Promoter misc_feature | 309 | 724 | 416 | + |
| Bar' | Selection marker CDS | 745 | 978 | 234 | + |
| STLS1 | Other misc_feature | 979 | 1,167 | 189 | + |
| ''Bar' | Selection marker CDS | 1,168 | 1,483 | 316 | + |
| 'E9 | Terminator misc_feature | 1,496 | 1,786 | 291 | + |
| Pro-Ubq10At | Promoter misc_feature | 1,835 | 3,310 | 1,476 | + |
| Ubi cds 5` | Coding sequence CDS | 2,939 | 3,002 | 64 | + |
| íntron | Other misc_feature | 3,005 | 3,308 | 304 | + |
| 'Cas9WT | Coding sequence CDS | 3,332 | 7,528 | 4,197 | + |
| NLS | Site misc_feature | 3,338 | 3,358 | 21 | + |
| NLS | Site misc_feature | 7,478 | 7,525 | 48 | + |
| NOS-T | Terminator misc_feature | 7,534 | 7,787 | 254 | + |
| 'RNA' | Other misc_feature | 7,792 | 8,352 | 561 | + |
| U6At | Coding sequence CDS | 7,797 | 8,241 | 445 | + |
| targetRNA | Site CDS | 8,242 | 8,268 | 27 | + |
| hairpin | Site CDS | 8,269 | 8,298 | 30 | + |
| gRNA | Site misc_feature | 8,269 | 8,351 | 83 | + |
| RB | T-DNA border misc_feature | 8,437 | 8,460 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 8,995 | 9,692 | 698 | + |
| 'pVS1 | Origin / backbone misc_feature | 9,980 | 12,767 | 2,788 | + |
| Sm/Sp | Selection marker CDS | 12,951 | 13,961 | 1,011 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.