p7onor-35SCasoi B815
Ligation of B400p7oN-LH* into B792p6ioR-35sCasWToi*
- Promoter 1
- Nos
- Gene 1
- bar
- Promoter 2
- 35s
- Gene 2
- Cas9
- Features
- U6At
- Size
- 13,313 bp, circular
- Record date
- 21-MAR-2016
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| LB | T-DNA border misc_feature | 1 | 24 | 24 | + |
| P-NOS | Other misc_feature | 309 | 606 | 298 | + |
| Bar' | Selection marker CDS | 625 | 858 | 234 | + |
| STLS1 | Other misc_feature | 859 | 1,047 | 189 | + |
| ''Bar' | Selection marker CDS | 1,048 | 1,363 | 316 | + |
| 'E9 | Terminator misc_feature | 1,376 | 1,666 | 291 | + |
| 35S-Pro | Promoter misc_feature | 1,721 | 2,138 | 418 | + |
| TATA-box | Site CDS | 2,109 | 2,115 | 7 | + |
| 'Cas9WT | Coding sequence CDS | 2,164 | 6,360 | 4,197 | + |
| NLS | Site misc_feature | 2,170 | 2,190 | 21 | + |
| NLS | Site misc_feature | 6,310 | 6,357 | 48 | + |
| NOS-T | Terminator misc_feature | 6,366 | 6,619 | 254 | + |
| 'RNA' | Other misc_feature | 6,624 | 7,184 | 561 | + |
| U6At | Coding sequence CDS | 6,629 | 7,073 | 445 | + |
| targetRNA | Site CDS | 7,074 | 7,100 | 27 | + |
| hairpin | Site CDS | 7,101 | 7,130 | 30 | + |
| gRNA | Site misc_feature | 7,101 | 7,183 | 83 | + |
| RB | T-DNA border misc_feature | 7,269 | 7,292 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 7,827 | 8,524 | 698 | + |
| 'pVS1 | Origin / backbone misc_feature | 8,812 | 11,599 | 2,788 | + |
| Sm/Sp | Selection marker CDS | 11,783 | 12,793 | 1,011 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.