p9ioR-35sCasDead D182

Ligation of dCas-Nru-Xma* into B358p9ioR-35sCasD10A*

Files

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Feature	Type	Start	End	Length	Strand
LB	T-DNA border misc_feature	1	24	24	+
P35S	Promoter misc_feature	309	724	416	+
NptII	Site CDS	741	777	37	+
int	Site misc_feature	778	966	189	+
NptII	Selection marker CDS	967	1,733	767	+
TE9	Terminator misc_feature	1,744	2,034	291	+
P35S	Promoter misc_feature	2,089	2,506	418	+
TATA-box	Site misc_feature	2,477	2,483	7	+
'Cas9	Coding sequence CDS	2,532	6,827	4,296	+
NLS	Site misc_feature	2,544	2,564	21	+
D10A	Site misc_feature	2,608	2,608	1	+
intron from rbcs Nicotiana tobaccum	Site misc_feature	2,955	3,047	93	+
H840A	Site misc_feature	5,190	5,191	2	+
NLS	Site misc_feature	6,777	6,824	48	+
NOS-T	Terminator misc_feature	6,833	7,086	254	+
U6At	Promoter misc_feature	7,096	7,540	445	+
targetRNA	Site CDS	7,541	7,567	27	+
hairpin	Site CDS	7,568	7,597	30	+
gRNA	Site misc_feature	7,568	7,650	83	+
RB	T-DNA border misc_feature	7,736	7,759	24	+
ColE1	Origin / backbone misc_feature	8,294	8,991	698	+
pVS1	Origin / backbone misc_feature	9,279	12,066	2,788	+
Sm/Sp	Selection marker CDS	12,250	13,260	1,011	+

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.