p9ioR-35sD10Aoi B763

Ligation of Cas-Bam-Nru* into B357p9ioR-35sCasWT*

Files

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Feature	Type	Start	End	Length	Strand
LB	T-DNA border misc_feature	1	24	24	+
35S	Promoter misc_feature	309	724	416	+
NptII	Site CDS	741	777	37	+
int	Site misc_feature	778	966	189	+
NptII	Selection marker CDS	967	1,733	767	+
'E9	Terminator misc_feature	1,744	2,034	291	+
35S-Pro	Promoter misc_feature	2,089	2,506	418	+
TATA-box	Site CDS	2,477	2,483	7	+
D10A	Coding sequence CDS	2,532	6,734	4,203	+
NLS	Site misc_feature	2,538	2,558	21	+
D10A	Site misc_feature	2,608	2,608	1	+
NLS	Site misc_feature	6,684	6,731	48	+
NOS-T	Terminator misc_feature	6,740	6,993	254	+
'RNA'	Other misc_feature	6,998	7,558	561	+
U6At	Coding sequence CDS	7,003	7,447	445	+
targetRNA	Site CDS	7,448	7,474	27	+
hairpin	Site CDS	7,475	7,504	30	+
gRNA	Site misc_feature	7,475	7,557	83	+
RB	T-DNA border misc_feature	7,643	7,666	24	+
ColE1	Origin / backbone misc_feature	8,201	8,898	698	+
'pVS1	Origin / backbone misc_feature	9,186	11,973	2,788	+
Sm/Sp	Selection marker CDS	12,157	13,167	1,011	+

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.