p9ioR-Uq10CasWToi C111

Ligation of B415p9o-LH* into C035p7ioR-Uq10CasWToi*

Files

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Feature	Type	Start	End	Length	Strand
LB	T-DNA border misc_feature	1	24	24	+
35S	Promoter misc_feature	309	724	416	+
NptIIo'	Selection marker CDS	745	1,043	299	+
splice	Site misc_feature	1,040	1,043	4	+
'intron	Coding sequence CDS	1,044	1,230	187	+
splice	Site misc_feature	1,229	1,232	4	+
''NptIIo'	Selection marker CDS	1,231	1,734	504	+
'E9	Terminator misc_feature	1,748	2,038	291	+
Pro-Ubq10At	Promoter misc_feature	2,087	3,562	1,476	+
Ubi cds 5`	Coding sequence CDS	3,191	3,254	64	+
íntron	Other misc_feature	3,257	3,560	304	+
'Cas9WT	Coding sequence CDS	3,584	7,780	4,197	+
NLS	Site misc_feature	3,590	3,610	21	+
NLS	Site misc_feature	7,730	7,777	48	+
NOS-T	Terminator misc_feature	7,786	8,039	254	+
'RNA'	Other misc_feature	8,044	8,604	561	+
U6At	Coding sequence CDS	8,049	8,493	445	+
targetRNA	Site CDS	8,494	8,520	27	+
hairpin	Site CDS	8,521	8,550	30	+
gRNA	Site misc_feature	8,521	8,603	83	+
RB	T-DNA border misc_feature	8,689	8,712	24	+
ColE1	Origin / backbone misc_feature	9,247	9,944	698	+
'pVS1	Origin / backbone misc_feature	10,232	13,019	2,788	+
Sm/Sp	Selection marker CDS	13,203	14,213	1,011	+

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.