p9oNoR-U10-Cpf1-U6At D915
Ligation of CpfU6-Bam-Kpn* into D922p9oNoR-U10*
- Promoter 1
- Nos
- Gene 1
- nptII
- Promoter 2
- Ubq10
- Gene 2
- LbCPf1
- Features
- Dicots
- Size
- 14,136 bp, circular
- Record date
- 26-AUG-2020
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| BL | Site misc_feature | 1 | 24 | 24 | + |
| PNOS | Promoter misc_feature | 309 | 606 | 298 | + |
| NptIIo | Selection marker CDS | 625 | 923 | 299 | + |
| int | Site CDS | 924 | 1,110 | 187 | + |
| NptIIo | Selection marker CDS | 1,111 | 1,614 | 504 | + |
| TE9 | Terminator misc_feature | 1,628 | 1,918 | 291 | + |
| Pro-Ubq10At | Promoter misc_feature | 1,967 | 3,442 | 1,476 | + |
| TATA | Site misc_feature | 3,038 | 3,041 | 4 | + |
| Ubi cds 5 | Promoter misc_feature | 3,071 | 3,134 | 64 | + |
| íntron | Other misc_feature | 3,137 | 3,440 | 304 | + |
| ORF-1 | Coding sequence CDS | 3,463 | 7,245 | 3,783 | + |
| NLS | Site misc_feature | 3,469 | 3,489 | 21 | + |
| NLS | Site misc_feature | 7,195 | 7,242 | 48 | + |
| NOS-T | Terminator misc_feature | 7,251 | 7,504 | 254 | + |
| U6At | Promoter misc_feature | 7,514 | 7,958 | 445 | + |
| DR | Site CDS | 7,959 | 7,980 | 22 | + |
| RB | T-DNA border misc_feature | 8,092 | 8,115 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 8,650 | 9,347 | 698 | + |
| pVS1 | Origin / backbone misc_feature | 9,635 | 12,422 | 2,788 | + |
| Sm/Sp | Selection marker CDS | 12,606 | 13,616 | 1,011 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.