p35s-LmEGFP D360
Ligation of GFP-Hind-Sal* into B629p35s-LGFP*
- Promoter 1
- 35s
- Gene 1
- mEGFP
- Features
- dicots and monocots
- Size
- 4,553 bp, circular
- Record date
- 26-AUG-2020
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| 35S-Pro | Promoter misc_feature | 106 | 528 | 423 | + |
| TATA | Site CDS | 494 | 500 | 7 | + |
| mEGFP | Coding sequence CDS | 627 | 1,005 | 379 | + |
| F64L/S65T - enhanced fluorescence | Site misc_feature | 816 | 821 | 6 | + |
| ST-LS1 | Coding sequence CDS | 1,006 | 1,194 | 189 | + |
| mEGFP | Coding sequence CDS | 1,195 | 1,532 | 338 | + |
| A206K - monomeric protein | Site misc_feature | 1,431 | 1,433 | 3 | + |
| OCS | Terminator misc_feature | 1,542 | 1,737 | 196 | + |
| Amp | Selection marker misc_feature | 2,553 | 3,413 | 861 | + |
| ColE1 | Origin / backbone misc_feature | 3,456 | 4,172 | 717 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.