DNA Cloning Service

pUC

pUbiAt-mEGFP E038

Ligation of D360p35s-LmEGFP* into B592pUbiAt-mGFPD-ocs* corrected

12345Pro-Ubq10AtAmpColE1mEGFPmEGFPíntronOCSST-LS1Ubi cds 5E038pUbiAt-mEGFP5,546 bp
Promoter 1
Ubq10
Gene 1
mEGFP
Features
dicots
Size
5,546 bp, circular
Record date
26-AUG-2020

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
Pro-Ubq10At Promoter misc_feature 461,5211,476 +
TATA Site misc_feature 1,1171,1204 +
Ubi cds 5 Promoter misc_feature 1,1501,21364 +
íntron Other misc_feature 1,2161,519304 +
mEGFP Coding sequence CDS 1,5471,925379 +
ST-LS1 Coding sequence CDS 1,9262,114189 +
mEGFP Coding sequence CDS 2,1152,452338 +
A206K - monomeric protein Site misc_feature 2,3512,3533 +
OCS Terminator misc_feature 2,4622,657196 +
Amp Selection marker misc_feature 3,4744,334861 +
ColE1 Origin / backbone CDS 4,3775,093717 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.