DNA Cloning Service

pUC

pUbiM-dmolgusI 986

Ligation of dmG1-GInt-dmG2* into 955pUbiM-dmoLgus*

12345''dmoLgusUbi-intAmpdmoLgus'IntronNOS'Intron986pUbiM-dmolgus5,572 bp
Promoter 2
UbiZm
Gene 2
oGus
Features
optimized GUS-intron for monocot transformation
Size
5,572 bp, circular
Record date
20-JUN-2013

Files

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Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
Ubi-int Promoter misc_feature 771,063987 +
Intron Site misc_feature 1,0641,583520 +
dmoLgus' Coding sequence CDS 1,6522,266615 +
'Intron Site misc_feature 2,2672,455189 +
''dmoLgus Coding sequence CDS 2,4563,6521,197 +
NOS Other misc_feature 3,7203,973254 +
Amp Selection marker CDS 4,0554,917863 +
ColE1 Site misc_feature 4,918100 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.