pmGFPD-nos B445
Ligation of GFPdi-Hind-Sal* into pNos-BM*
- Promoter 2
- MCS
- Gene 2
- GFP
- Features
- dicots
- Size
- 4,150 bp, circular
- Record date
- 22-JAN-2016
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| gfp | Coding sequence CDS | 179 | 557 | 379 | + |
| int | Site CDS | 558 | 746 | 189 | + |
| gfp | Coding sequence CDS | 747 | 1,084 | 338 | + |
| Tnos | Terminator misc_feature | 1,106 | 1,359 | 254 | + |
| Amp | Selection marker misc_feature | 2,176 | 3,036 | 861 | + |
| ColE1 | Origin / backbone CDS | 3,079 | 3,795 | 717 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.