DNA Cloning Service

RNAi

p1U-SG

Ligation of Lox-Ubi-SG* into pD1AF*

1234Ubi-intAmpSGIntronNOSp1U-SG4,255 bp
Promoter 2
Ubi-maize
Gene 2
Spacer-Gus
Features
hairpin construct with spacer, for RNAi in monocots
Size
4,255 bp, circular
Record date
13-JUL-2010

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
Ubi-int Promoter misc_feature 781,5861,509 +
Intron Site misc_feature 1,0651,584520 +
SG Other misc_feature 1,5972,369773 +
NOS Other misc_feature 2,4032,656254 +
Amp Selection marker CDS 2,7383,600863 +
ColE1 Site misc_feature 3,601100 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.