p6-Ubi-RNAi2
Ligation of inverted A287pD1-Ubi-RNAi2* into pLH6000*
- Promoter 1
- 35S
- Gene 1
- hpt
- Promoter 2
- Ubi-maize
- Gene 2
- gus-spacer
- Features
- double promoter construct for use in monocots
- Size
- 13,050 bp, circular
- Record date
- 15-FEB-2013
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| 35S | Promoter misc_feature | 7 | 422 | 416 | + |
| hpt | Selection marker CDS | 452 | 1,477 | 1,026 | + |
| T35S | Terminator misc_feature | 1,502 | 1,703 | 202 | + |
| 'Ubi-int' | Promoter misc_feature | 1,763 | 3,238 | 1,476 | + |
| Intron | Site misc_feature | 2,717 | 3,236 | 520 | + |
| T35s | Terminator misc_feature | 3,252 | 3,300 | 49 | − |
| unnamed | Coding sequence CDS | 3,320 | 3,869 | 550 | + |
| Gus | Coding sequence CDS | 3,320 | 3,869 | 550 | − |
| T35S | Terminator misc_feature | 3,890 | 3,938 | 49 | + |
| 'Ubi-int' | Promoter misc_feature | 3,952 | 5,427 | 1,476 | − |
| Intron | Site misc_feature | 3,954 | 4,473 | 520 | − |
| BR | Site misc_feature | 5,525 | 5,548 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 6,083 | 6,780 | 698 | + |
| bom | Origin / backbone misc_feature | 6,817 | 7,076 | 260 | + |
| resolvase | Other misc_feature | 7,456 | 8,142 | 687 | + |
| ParA | Terminator misc_feature | 8,441 | 9,070 | 630 | + |
| ParB | Terminator misc_feature | 9,397 | 10,572 | 1,176 | + |
| Sm/Sp | Selection marker CDS | 11,218 | 12,228 | 1,011 | + |
| BL | Site misc_feature | 12,749 | 12,772 | 24 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.