p6N-35S-Ubi10-RNAi
Ligation of inverted 670pD1-35s-Ubi10-RNAi* into p6N*
- Promoter 1
- Nos
- Gene 1
- hpt
- Promoter 2
- 35S
- Gene 2
- gus-spacer
- Features
- double promoter construct for use in dicots (Arabidopsis+Tobacco)
- Size
- 11,964 bp, circular
- Record date
- 20-JUN-2013
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| Pnos | Promoter misc_feature | 7 | 304 | 298 | + |
| hptII | Selection marker CDS | 332 | 1,357 | 1,026 | + |
| T35S | Terminator misc_feature | 1,382 | 1,583 | 202 | + |
| Pro-35S | Promoter misc_feature | 1,642 | 2,154 | 513 | + |
| T35s | Terminator misc_feature | 2,168 | 2,216 | 49 | − |
| unnamed | Coding sequence CDS | 2,236 | 2,785 | 550 | + |
| Gus | Coding sequence CDS | 2,236 | 2,785 | 550 | − |
| T35S | Terminator misc_feature | 2,806 | 2,854 | 49 | + |
| Ubq10At | Other misc_feature | 2,868 | 4,343 | 1,476 | − |
| intron | Site misc_feature | 2,870 | 3,173 | 304 | − |
| Ubi cds 5` | Site misc_signal | 3,176 | 3,239 | 64 | − |
| BR | Site misc_feature | 4,439 | 4,462 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 4,997 | 5,694 | 698 | + |
| pVS1 ORI | Origin / backbone misc_feature | 6,243 | 9,948 | 3,706 | + |
| Sm/Sp | Selection marker CDS | 10,132 | 11,142 | 1,011 | + |
| BL | Site misc_feature | 11,663 | 11,686 | 24 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.