p6mD-Ubi-RNAi
Ligation of pUbi-iF2* into 611p6moD35*
- Promoter 1
- 35s2x
- Gene 1
- hpt-intron-mo
- Promoter 2
- ubi-maize
- Gene 2
- iF2
- Features
- hairpin construct with intron, for RNAi in monocots, codon optimized hptII for monocots
- Size
- 12,904 bp, circular
- Record date
- 20-JUN-2013
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| P35s2X | Promoter misc_feature | 67 | 814 | 748 | + |
| hptII | Selection marker CDS | 871 | 1,440 | 570 | + |
| int | Site misc_feature | 1,441 | 1,629 | 189 | + |
| 'hptII | Selection marker CDS | 1,630 | 2,085 | 456 | + |
| E9 | Terminator misc_feature | 2,096 | 2,386 | 291 | + |
| UbiZm | Promoter misc_feature | 2,441 | 3,949 | 1,509 | + |
| iF2 | Other misc_feature | 3,987 | 5,118 | 1,132 | + |
| ocs | Terminator misc_feature | 5,153 | 5,348 | 196 | + |
| RB | T-DNA border misc_feature | 5,433 | 5,456 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 5,991 | 6,688 | 698 | + |
| pVS1 | Origin / backbone misc_feature | 7,237 | 10,942 | 3,706 | + |
| Sm/Sp | Selection marker CDS | 11,126 | 12,136 | 1,011 | + |
| LB | T-DNA border misc_feature | 12,657 | 12,680 | 24 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.