p7N-35S-Ubi10-RNAi
Ligation of inverted 670pD1-35s-Ubi10-RNAi* into p7N*
- Promoter 1
- Nos
- Gene 1
- bar
- Promoter 2
- 35S
- Gene 2
- gus-spacer
- Features
- double promoter construct for use in dicots (Arabidopsis+Tobacco)
- Size
- 11,474 bp, circular
- Record date
- 12-JUL-2013
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| Pnos | Promoter misc_feature | 7 | 304 | 298 | + |
| bar | Selection marker misc_feature | 319 | 870 | 552 | + |
| T35S | Terminator misc_feature | 893 | 1,093 | 201 | + |
| P35S | Promoter misc_feature | 1,152 | 1,664 | 513 | + |
| T35s | Terminator misc_feature | 1,678 | 1,726 | 49 | − |
| unnamed | Coding sequence CDS | 1,746 | 2,295 | 550 | + |
| Gus | Coding sequence CDS | 1,746 | 2,295 | 550 | − |
| T35S | Terminator misc_feature | 2,316 | 2,364 | 49 | + |
| Ubq10At | Other misc_feature | 2,378 | 3,853 | 1,476 | − |
| íntron | Other misc_feature | 2,380 | 2,683 | 304 | − |
| RB | T-DNA border misc_feature | 3,949 | 3,972 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 4,507 | 5,204 | 698 | + |
| pVS1 ORI | Origin / backbone misc_feature | 5,753 | 9,458 | 3,706 | + |
| Sm/Sp | Selection marker CDS | 9,642 | 10,652 | 1,011 | + |
| LB | T-DNA border misc_feature | 11,173 | 11,196 | 24 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.