p7U-35S-RNAi
Ligation of inverted 1.pD1-35s-RNAi* into p7U*
- Promoter 1
- Ubi-maize
- Gene 1
- bar
- Promoter 2
- 35S
- Gene 2
- gus-spacer
- Features
- double promoter construct for use in monocots
- Size
- 11,637 bp, circular
- Record date
- 13-OCT-2009
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| Ubi-int' | Promoter misc_feature | 10 | 1,517 | 1,508 | + |
| Intron | Site misc_feature | 997 | 1,516 | 520 | + |
| bar | Selection marker CDS | 1,531 | 2,082 | 552 | + |
| T35S | Terminator misc_feature | 2,105 | 2,305 | 201 | + |
| Pro-35S | Promoter misc_feature | 2,364 | 2,876 | 513 | + |
| T35s | Site misc_feature | 0 | 0 | 1 | − |
| (null) | Coding sequence CDS | 2,958 | 3,507 | 550 | + |
| Gus | Site CDS | 0 | 0 | 1 | − |
| T35S | Terminator misc_feature | 3,528 | 3,576 | 49 | + |
| Pro-35S | Site misc_feature | 0 | 0 | 1 | − |
| RB | T-DNA border misc_feature | 4,112 | 4,135 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 4,670 | 5,367 | 698 | + |
| pVS1 ORI | Origin / backbone misc_feature | 5,916 | 9,621 | 3,706 | + |
| Sm/Sp | Selection marker CDS | 9,805 | 10,815 | 1,011 | + |
| LB | T-DNA border misc_feature | 11,336 | 11,359 | 24 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.