DNA Cloning Service

RNAi

p7U10-RNAi

Ligation of inverted pD1-35s-RNAi* into p7U10*

1234567891011pVS1 ORIUBQ10Sm/SpColE1bar(null)Pro-35SíntronT35ST35SRBLBp7U10-RNAi11,610 bp
Promoter 1
UBQ10AT
Gene 1
bar
Promoter 2
35S
Gene 2
gus-spacer
Features
double promoter construct for dicots
Size
11,610 bp, circular
Record date
09-OCT-2009

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
UBQ10 Other misc_feature 71,4841,478 +
íntron Other misc_feature 1,1791,482304 +
bar Selection marker CDS 1,5042,055552 +
T35S Terminator misc_feature 2,0782,278201 +
Pro-35S Promoter misc_feature 2,3372,849513 +
T35s Site misc_feature 001 −
(null) Coding sequence CDS 2,9313,480550 +
Gus Site CDS 001 −
T35S Terminator misc_feature 3,5013,54949 +
Pro-35S Site misc_feature 001 −
RB T-DNA border misc_feature 4,0854,10824 +
ColE1 Origin / backbone misc_feature 4,6435,340698 +
pVS1 ORI Origin / backbone misc_feature 5,8899,5943,706 +
Sm/Sp Selection marker CDS 9,77810,7881,011 +
LB T-DNA border misc_feature 11,30911,33224 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.