p7i-Ubi-RNAi2
Ligation of inverted A287pD1-Ubi-RNAi2* into p7iM*
- Promoter 1
- 35S
- Gene 1
- bar
- Promoter 2
- Ubi-maize
- Gene 2
- gus-spacer
- Features
- double promoter construct for use in monocots
- Size
- 12,749 bp, circular
- Record date
- 14-MAR-2013
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| 35S | Promoter misc_feature | 7 | 422 | 416 | + |
| bar | Selection marker CDS | 439 | 672 | 234 | + |
| STLS1 | Coding sequence CDS | 673 | 861 | 189 | + |
| bar | Selection marker CDS | 862 | 1,179 | 318 | + |
| T35S | Terminator misc_feature | 1,201 | 1,402 | 202 | + |
| 'Ubi-int' | Promoter misc_feature | 1,462 | 2,937 | 1,476 | + |
| Intron | Site misc_feature | 2,416 | 2,935 | 520 | + |
| T35s | Terminator misc_feature | 2,951 | 2,999 | 49 | − |
| unnamed | Coding sequence CDS | 3,019 | 3,568 | 550 | + |
| Gus | Coding sequence CDS | 3,019 | 3,568 | 550 | − |
| T35S | Terminator misc_feature | 3,589 | 3,637 | 49 | + |
| 'Ubi-int' | Promoter misc_feature | 3,651 | 5,126 | 1,476 | − |
| Intron | Site misc_feature | 3,653 | 4,172 | 520 | − |
| RB | T-DNA border misc_feature | 5,224 | 5,247 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 5,782 | 6,479 | 698 | + |
| pVS1 ORI | Origin / backbone misc_feature | 7,028 | 10,733 | 3,706 | + |
| Sm/Sp | Selection marker CDS | 10,917 | 11,927 | 1,011 | + |
| LB | T-DNA border misc_feature | 12,448 | 12,471 | 24 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.