p7iUA-RNAi
Ligation of inverted 589pD1-Ubi-Act-RNAi* into p7i*
- Promoter 1
- 35S
- Gene 1
- bar-intron
- Promoter 2
- Act
- Gene 2
- gus-spacer
- Features
- double promoter construct for use in monocots
- Size
- 12,671 bp, circular
- Record date
- 20-JUN-2013
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| 35S | Promoter misc_feature | 7 | 422 | 416 | + |
| bar | Selection marker misc_feature | 439 | 672 | 234 | + |
| int | Site misc_feature | 673 | 861 | 189 | + |
| bar | Selection marker misc_feature | 862 | 1,179 | 318 | + |
| T35S | Terminator misc_feature | 1,201 | 1,402 | 202 | + |
| PAct | Promoter misc_feature | 1,461 | 2,858 | 1,398 | + |
| T35s | Terminator misc_feature | 2,876 | 2,924 | 49 | − |
| unnamed | Coding sequence CDS | 2,944 | 3,493 | 550 | + |
| Gus | Coding sequence CDS | 2,944 | 3,493 | 550 | − |
| T35S | Terminator misc_feature | 3,514 | 3,562 | 49 | + |
| Ubi-int | Promoter misc_feature | 3,575 | 5,050 | 1,476 | − |
| Intron UbiZm | Promoter misc_feature | 3,577 | 4,096 | 520 | − |
| RB | T-DNA border misc_feature | 5,146 | 5,169 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 5,704 | 6,401 | 698 | + |
| pVS1 ORI | Origin / backbone misc_feature | 6,950 | 10,655 | 3,706 | + |
| Sm/Sp | Selection marker CDS | 10,839 | 11,849 | 1,011 | + |
| LB | T-DNA border misc_feature | 12,370 | 12,393 | 24 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.