p9-Nap-RNAi
Ligation of inverted 2.pD1-Nap-RNAi* into pLH9000*
- Promoter 1
- 35S
- Gene 1
- npt
- Promoter 2
- Napin
- Gene 2
- gus-spacer
- Features
- double promoter construct for use in rapeseed
- Size
- 10,588 bp, circular
- Record date
- 25-JUN-2010
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| 35S | Promoter misc_feature | 7 | 422 | 416 | + |
| NptII | Selection marker CDS | 439 | 1,242 | 804 | + |
| T35S | Terminator misc_feature | 1,255 | 1,456 | 202 | + |
| Nap | Other misc_feature | 1,517 | 1,880 | 364 | + |
| T35s | Site misc_feature | 0 | 0 | 1 | − |
| (null) | Coding sequence CDS | 1,967 | 2,516 | 550 | + |
| Gus | Site CDS | 0 | 0 | 1 | − |
| T35S | Terminator misc_feature | 2,537 | 2,585 | 49 | + |
| Nap | Site misc_feature | 0 | 0 | 1 | − |
| BR | Site misc_feature | 3,063 | 3,086 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 3,621 | 4,318 | 698 | + |
| bom | Origin / backbone misc_feature | 4,355 | 4,614 | 260 | + |
| pVS1 | Origin / backbone misc_feature | 4,867 | 8,572 | 3,706 | + |
| Sm/Sp | Selection marker CDS | 8,756 | 9,766 | 1,011 | + |
| BL | Site misc_feature | 10,287 | 10,310 | 24 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.