p9-Ubi-RNAi2
Ligation of inverted A287pD1-Ubi-RNAi2* into pLH9000*
- Promoter 1
- 35S
- Gene 1
- npt
- Promoter 2
- Ubi-maize
- Gene 2
- gus-spacer
- Features
- double promoter construct for use in monocots
- Size
- 12,803 bp, circular
- Record date
- 13-FEB-2013
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| P35S | Promoter misc_feature | 7 | 422 | 416 | + |
| NptII | Selection marker CDS | 439 | 1,242 | 804 | + |
| T35S | Terminator misc_feature | 1,255 | 1,456 | 202 | + |
| 'Ubi-int' | Promoter misc_feature | 1,516 | 2,991 | 1,476 | + |
| Intron | Site misc_feature | 2,470 | 2,989 | 520 | + |
| T35s | Terminator misc_feature | 3,005 | 3,053 | 49 | − |
| Gus | Coding sequence CDS | 3,073 | 3,622 | 550 | + |
| Gus | Coding sequence CDS | 3,073 | 3,622 | 550 | − |
| T35S | Terminator misc_feature | 3,643 | 3,691 | 49 | + |
| 'Ubi-int' | Promoter misc_feature | 3,705 | 5,180 | 1,476 | − |
| Intron | Site misc_feature | 3,707 | 4,226 | 520 | − |
| RB | T-DNA border misc_feature | 5,278 | 5,301 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 5,836 | 6,533 | 698 | + |
| pVS1 | Origin / backbone misc_feature | 7,082 | 10,787 | 3,706 | + |
| corrected sequence, not g | Site misc_signal | 7,465 | 7,465 | 1 | + |
| Sm/Sp | Selection marker misc_feature | 10,971 | 11,981 | 1,011 | + |
| LB | T-DNA border misc_feature | 12,502 | 12,525 | 24 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.