DNA Cloning Service

RNAi

p9U10-RNAi

Ligation of inverted pD1-35s-RNAi* into p9U10*

1234567891011pVS1 ORIUBQ10Sm/SpNptIIColE1(null)Pro-35SintronT35ST35Sp9U10-RNAi11,853 bp
Promoter 1
UBQ10AT
Gene 1
npt
Promoter 2
35S
Gene 2
gus-spacer
Features
double promoter construct for dicots
Size
11,853 bp, circular
Record date
09-OCT-2009

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
UBQ10 Other misc_feature 71,4841,478 +
intron Site misc_feature 1,1791,482304 +
NptII Selection marker CDS 1,5042,307804 +
T35S Terminator misc_feature 2,3202,521202 +
Pro-35S Promoter misc_feature 2,5803,092513 +
T35s Site misc_feature 001
(null) Coding sequence CDS 3,1743,723550 +
Gus Site CDS 001
T35S Terminator misc_feature 3,7443,79249 +
Pro-35S Site misc_feature 001
BR Site misc_feature 4,3284,35124 +
ColE1 Origin / backbone misc_feature 4,8865,583698 +
pVS1 ORI Origin / backbone misc_feature 6,1329,8373,706 +
Sm/Sp Selection marker CDS 10,02111,0311,011 +
BL Site misc_feature 11,55211,57524 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.