p9i-UbiH-2xStp-RNAi
Ligation of inverted A252pD1-UbiH-2xStp-RNAi* into p9i*
- Promoter 1
- 35S
- Gene 1
- npt-intron
- Promoter 2
- GUBB1HA
- Gene 2
- gus-spacer
- Features
- double promoter construct GUBB1HA with improved termination signals
- Size
- 10,741 bp, circular
- Record date
- 20-JUN-2013
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| 35S | Promoter misc_feature | 7 | 422 | 416 | + |
| NptII | Site CDS | 439 | 475 | 37 | + |
| int | Site misc_feature | 476 | 664 | 189 | + |
| NptII | Selection marker CDS | 665 | 1,431 | 767 | + |
| T-35S | Promoter misc_feature | 1,444 | 1,645 | 202 | + |
| UbiH | Promoter misc_feature | 1,704 | 2,047 | 344 | + |
| T35S | Terminator misc_feature | 2,062 | 2,114 | 53 | − |
| unnamed | Coding sequence CDS | 2,134 | 2,683 | 550 | + |
| Gus | Coding sequence CDS | 2,134 | 2,683 | 550 | − |
| T35S | Terminator misc_feature | 2,704 | 2,756 | 53 | + |
| UbiH | Promoter misc_feature | 2,771 | 3,114 | 344 | − |
| BR | Site misc_feature | 3,216 | 3,239 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 3,774 | 4,471 | 698 | + |
| pVS1 ORI | Origin / backbone misc_feature | 5,020 | 8,725 | 3,706 | + |
| Sm/Sp | Selection marker CDS | 8,909 | 9,919 | 1,011 | + |
| BL | Site misc_feature | 10,440 | 10,463 | 24 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.