DNA Cloning Service

RNAi

pD1-35s-Ubi10-RNAi

Ligation of 204pUbiAtmu-ocs* into pD1-35s-RNAi*

1234Pro-Ubq10AtAmpGusPro-35SintUbi cds 5`T35ST35spD1-35s-Ubi10-RN4,372 bp
Promoter 2
UBQ10AT
Gene 2
gus-spacer
Features
double promoter construct UBQ10AT+35S for dicots
Size
4,372 bp, circular
Record date
20-JUN-2013

Files

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Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
Pro-Ubq10At Promoter misc_feature 711,5461,476 +
Ubi cds 5` Coding sequence CDS 1,1751,23864 +
int Site misc_feature 1,2411,544304 +
T35S Terminator misc_feature 1,5601,60849
Gus Coding sequence CDS 1,6292,178550 +
unnamed Coding sequence CDS 1,6292,178550
T35s Terminator misc_feature 2,1982,24649 +
Pro-35S Promoter misc_feature 2,2602,772513
Amp Selection marker CDS 2,8553,717863 +
ColE1 Site misc_feature 3,718100 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.