DNA Cloning Service

RNAi

pD1-Ubi-Act-RNAi

Ligation of inverted 153pD1-Act-RNAi* into 46pD1-Ubi-L-RNAi*

12345UbiZmpActAmpGusintT35ST35spD1-Ubi-Act-RNAi5,260 bp
Promoter 2
ubi-maize
Gene 2
gus-spacer
Features
double promoter construct ubi-maize+Act for monocots
Size
5,260 bp, circular
Record date
20-JUN-2013

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
UbiZm Promoter misc_feature 711,5461,476 +
int Site misc_feature 1,0251,544520 +
T35S Terminator misc_feature 1,5591,60749
Gus Coding sequence CDS 1,6282,177550 +
unnamed Coding sequence CDS 1,6282,177550
T35s Terminator misc_feature 2,1972,24549 +
pAct Promoter misc_feature 2,2633,6601,398
Amp Selection marker CDS 3,7434,605863 +
ColE1 Site misc_feature 4,606100 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.