pD1-Ubi-RNAI2
Ligation of inverted Ubi-Spe-Eag* into 44pD1-35s-Ubi-RNAi*
- Promoter 2
- Ubi-maize
- Gene 2
- gus-spacer
- Features
- double promoter construct Ubi-maize
- Size
- 5,338 bp, circular
- Record date
- 13-FEB-2013
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| 'Ubi-int' | Coding sequence CDS | 73 | 1,548 | 1,476 | + |
| Intron | Site misc_feature | 1,027 | 1,546 | 520 | + |
| T35S | Terminator misc_feature | 1,562 | 1,610 | 49 | − |
| Gus | Coding sequence CDS | 1,631 | 2,180 | 550 | + |
| unnamed | Coding sequence CDS | 1,631 | 2,180 | 550 | − |
| T35s | Terminator misc_feature | 2,200 | 2,248 | 49 | + |
| 'Ubi-int' | Coding sequence CDS | 2,262 | 3,737 | 1,476 | − |
| Intron | Site misc_feature | 2,264 | 2,783 | 520 | − |
| Amp | Selection marker CDS | 3,821 | 4,683 | 863 | + |
| ColE1 | Site misc_feature | 4,684 | 10 | 0 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.