DNA Cloning Service

RNAi

pLNU-SG

Ligation of Spacer-Gus into Lox-Ubi-Nos

1234Ubi-intSG-GliIntronNOSpLNU-SG4,495 bp
Promoter 2
Ubi-maize
Gene 2
Spacer-Gus
Features
hairpin construct with spacer, Lox sites in cassette
Size
4,495 bp, circular
Record date
13-JUL-2010

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
loxP Site misc_feature 134634 +
Ubi-int Promoter misc_feature 621,5701,509 +
Intron Site misc_feature 1,0491,568520 +
SG-Gli Other misc_feature 1,5812,353773 +
NOS Other misc_feature 2,3872,640254 +
LoxP Site misc_feature 2,6472,68034 +
ColE1 Site misc_feature 001
Amp Site CDS 001

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.