DNA Cloning Service

Binaries

p5o2x35s-LH B482

Ligation of 35s-Xba-xho* into B472p5oM-LH*

12345678910pVS1Sm/SpepspsP2x35sEPSPSColE1TE9intLBRBB482p5o2x35s-LH10,587 bp
Promoter 1
2x35s
Gene 1
EPSPS
Features
monocot
Size
10,587 bp, circular
Record date
22-JAN-2016

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
LB T-DNA border misc_feature 12424 +
P2x35s Promoter misc_feature 3141,098785 +
EPSPS Coding sequence CDS 1,1161,856741 +
int Site misc_feature 1,8572,045189 +
epsps Coding sequence CDS 2,0462,888843 +
TE9 Terminator misc_feature 2,8983,188291 +
RB T-DNA border misc_feature 3,3643,38724 +
ColE1 Origin / backbone misc_feature 3,9224,619698 +
pVS1 Origin / backbone misc_feature 5,1688,8733,706 +
Sm/Sp Selection marker CDS 9,05710,0671,011 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.