DNA Cloning Service

Binaries

p5oU10-2 B638

Ligation of esps-Xho-Mlu* into B435p9oU10-LH*

1234567891011pVS1UBQ10Sm/SpepspsColE1EPSPSintTE9bomcptintUbi cds 5RBLBB638p5oU10-211,287 bp
Promoter 1
Ubq10
Gene 1
EPSPS
Features
dicots
Size
11,287 bp, circular
Record date
08-FEB-2016

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
LB T-DNA border misc_feature 12424 +
UBQ10 Other misc_feature 3091,7841,476 +
Ubi cds 5 Promoter misc_feature 1,4131,47664 +
int Site misc_feature 1,4791,782304 +
cpt Coding sequence CDS 1,8042,022219 +
EPSPS Coding sequence CDS 2,0232,556534 +
int Site misc_feature 2,5572,745189 +
epsps Coding sequence CDS 2,7463,588843 +
TE9 Terminator misc_feature 3,5983,888291 +
RB T-DNA border misc_feature 4,0644,08724 +
ColE1 Origin / backbone misc_feature 4,6225,319698 +
bom Origin / backbone misc_feature 5,3565,615260 +
pVS1 Origin / backbone misc_feature 5,8689,5733,706 +
Sm/Sp Selection marker CDS 9,75710,7671,011 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.