DNA Cloning Service

Binaries

p6o-LH-35s-ocs B423

Ligation of p35-OCS-BM* into B413p6o-LH*

12345678910pVS1Sm/SpColE1hptIIhptIIP35SP35STE9bomTocsintLBRBB423p6o-LH-35s-o10,287 bp
Promoter 1
35s
Gene 1
hptII
Promoter 2
35s
Gene 2
MCS
Features
dicots
Size
10,287 bp, circular
Record date
21-JAN-2016

Files

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Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
LB T-DNA border misc_feature 12424 +
P35S Promoter misc_feature 309724416 +
hptII Selection marker CDS 7451,314570 +
int Site misc_feature 1,3151,503189 +
hptII Selection marker CDS 1,5041,959456 +
TE9 Terminator misc_feature 1,9702,260291 +
P35S Promoter misc_feature 2,3152,737423 +
Tocs Terminator misc_feature 2,7842,979196 +
RB T-DNA border misc_feature 3,0643,08724 +
ColE1 Origin / backbone misc_feature 3,6224,319698 +
bom Origin / backbone misc_feature 4,3564,615260 +
pVS1 Origin / backbone misc_feature 4,8688,5733,706 +
Sm/Sp Selection marker CDS 8,7579,7671,011 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.