DNA Cloning Service

Binaries

p7o-LH-35s-ocs B424

Ligation of p35-OCS-BM* into B414p7o-LH*

123456789pVS1Sm/SpColE1P35SP35SbarTE9bombarTocsintLBRBB424p7o-LH-35s-o9,813 bp
Promoter 1
35s
Gene 1
bar
Promoter 2
35s
Gene 2
MCS
Features
dicots
Size
9,813 bp, circular
Record date
22-JAN-2016

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
LB T-DNA border misc_feature 12424 +
P35S Promoter misc_feature 309724416 +
bar Selection marker CDS 745978234 +
int Site misc_feature 9791,167189 +
bar Selection marker CDS 1,1681,483316 +
TE9 Terminator misc_feature 1,4961,786291 +
P35S Promoter misc_feature 1,8412,263423 +
Tocs Terminator misc_feature 2,3102,505196 +
RB T-DNA border misc_feature 2,5902,61324 +
ColE1 Origin / backbone misc_feature 3,1483,845698 +
bom Origin / backbone misc_feature 3,8824,141260 +
pVS1 Origin / backbone misc_feature 4,3948,0993,706 +
Sm/Sp Selection marker CDS 8,2839,2931,011 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.